Ribonuclease A Solution (ES1061)

Ribonuclease A (10mg/ml, Solution), DNase and protease-free
Cat# ES1061 1ml
Source: Bovine pancreas.
Grade: PCR grade, for Molecular Biology
Storage Temperature -20° C

Description:
The RNase A(Ribonuclease A), DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate. The working concentration for RNase A is 1-100 µ G/ml depending on the application.
Applications
Plasmid and genomic DNA preparation.
Removal of RNA from recombinant protein preparations.
Ribonuclease protection assays.
Mapping single-base mutations in DNA or RNA.
Concentration: 10 mg/ml.
Specific Activity
≥ 70 Kunitz units/mg protein.

Note:
The working concentration for RNase A is 1-100 µ G/ml depending on the application.
The enzyme is active under a wide range of reaction conditions. At low salt concentrations (0 to 100 mM NaCl), RNase A cleaves single-stranded and double-stranded RNA as well the RNA strand in RNA-DNA hybrids. However, at NaCl concentrations of 0.3 M or higher, RNase A specifically cleaves single-stranded RNA.